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T lymphocyte proliferation evaluated by flow cytometry. ( A ) Percentage of CD3 + <t>CD4</t> + cells; ( B ) Percentage of CD3 + CD8 + cells, from dogs fed control diet and experimental diet, that proliferated at least once when non-stimulated (None) or in response to recombinant antigen from Leptospira interrogans (LipL32) and concanavalin A (ConA). Bars correspond to mean plus standard error of the mean. * P < 0.05.
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T lymphocyte proliferation evaluated by flow cytometry. ( A ) Percentage of CD3 + <t>CD4</t> + cells; ( B ) Percentage of CD3 + CD8 + cells, from dogs fed control diet and experimental diet, that proliferated at least once when non-stimulated (None) or in response to recombinant antigen from Leptospira interrogans (LipL32) and concanavalin A (ConA). Bars correspond to mean plus standard error of the mean. * P < 0.05.
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T lymphocyte proliferation evaluated by flow cytometry. ( A ) Percentage of CD3 + CD4 + cells; ( B ) Percentage of CD3 + CD8 + cells, from dogs fed control diet and experimental diet, that proliferated at least once when non-stimulated (None) or in response to recombinant antigen from Leptospira interrogans (LipL32) and concanavalin A (ConA). Bars correspond to mean plus standard error of the mean. * P < 0.05.

Journal: Scientific Reports

Article Title: Unraveling the role of shrimp hydrolysate as a food supplement in the immune function and fecal microbiota of beagle dogs

doi: 10.1038/s41598-025-09942-8

Figure Lengend Snippet: T lymphocyte proliferation evaluated by flow cytometry. ( A ) Percentage of CD3 + CD4 + cells; ( B ) Percentage of CD3 + CD8 + cells, from dogs fed control diet and experimental diet, that proliferated at least once when non-stimulated (None) or in response to recombinant antigen from Leptospira interrogans (LipL32) and concanavalin A (ConA). Bars correspond to mean plus standard error of the mean. * P < 0.05.

Article Snippet: The PBMC were washed with PBS and stained with anti-dog CD3 FITC-conjugate (Bio-Rad), anti-dog CD4 PE-Cy7-conjugate (eBioscience), and anti-dog CD25 Super Bright 436-conjugate (clone P4A10, 62-0250-42, eBioscience), protected from light, for 25 min at 4 °C.

Techniques: Flow Cytometry, Control, Recombinant

Intracellular cytokine measurement by flow cytometry. ( A ) Percentage of CD3 + CD4 + cells expressing interferon-gamma (IFN-γ), tumor necrosis factor-alpha (TNF-α) and both cytokines; ( B ) Percentage of CD3 + CD8 + cells expressing IFN-γ, TNF-α and both cytokines; ( C ) Ratio of CD3 + CD4 + and CD3 + CD8 + cells calculated from the percentage of CD4 + and CD8 + T cells; ( D ) Percentage of CD3 + CD4 + CD25 + cells expressing Foxp3 from dogs fed control diet and experimental diet. Bars correspond to mean plus standard error of the mean. ** P < 0.01.

Journal: Scientific Reports

Article Title: Unraveling the role of shrimp hydrolysate as a food supplement in the immune function and fecal microbiota of beagle dogs

doi: 10.1038/s41598-025-09942-8

Figure Lengend Snippet: Intracellular cytokine measurement by flow cytometry. ( A ) Percentage of CD3 + CD4 + cells expressing interferon-gamma (IFN-γ), tumor necrosis factor-alpha (TNF-α) and both cytokines; ( B ) Percentage of CD3 + CD8 + cells expressing IFN-γ, TNF-α and both cytokines; ( C ) Ratio of CD3 + CD4 + and CD3 + CD8 + cells calculated from the percentage of CD4 + and CD8 + T cells; ( D ) Percentage of CD3 + CD4 + CD25 + cells expressing Foxp3 from dogs fed control diet and experimental diet. Bars correspond to mean plus standard error of the mean. ** P < 0.01.

Article Snippet: The PBMC were washed with PBS and stained with anti-dog CD3 FITC-conjugate (Bio-Rad), anti-dog CD4 PE-Cy7-conjugate (eBioscience), and anti-dog CD25 Super Bright 436-conjugate (clone P4A10, 62-0250-42, eBioscience), protected from light, for 25 min at 4 °C.

Techniques: Flow Cytometry, Expressing, Control